Zero Chrom® Plasmid Platform – Innovative Robust Technology

 Pioneering Plasmid Manufacturing: A Time and Money-Saving Platform Making History

Robust, Innovative Zero Chrom® Plasmid Platform

  • Faster Purification: No column chromatography needed.
  • Low regulatory risk: Single use systems reduce contamination risk.
  • High-Quality Plasmid: > 95% supercoiled.
  • FDA & NMPA Approved: Zero Chrom plasmids gained IND approval.
  • High-Throughput: Over 300 batches per year.
  • Scalable Production: 1L-30L, research & GMP grades.
Production

For more information, connect with our experts by clicking on the contact us tab below.

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Plasmid Production Timeline

Zero Chrom Plasmid are available off-of-the-shelf in both PD and GMP grades

  • AAV Helper and Rep-CapX Plasmids
  • LVV helper and packaging plasmids
  • GFP and Luciferase plasmids
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Off-the-shelf Plasmid

CoJourney has off-the-shelf plasmids available to purchase. These research-grade plasmids are available as per your needs from milligram to gram scale.

Plasmids Availability

For more information on the quote on the off-the-shelf plasmids, click the tab below.

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Our Services

Robust, Compliant Plasmid Manufacturing

CoJourney’s revolutionary Zero Chrom Plasmid Platform was developed by process experts to transform plasmid manufacturing, enhancing quality and reducing costs.

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Upstream Process Development and Manufacturing

CoJourney’s industry-leading platform is supported by 5 production lines that generate over 350+ batches per year.

Process Development

  • E. coli plasmid
  • Clone selection and PCB banking
  • Expertise in fermentation optimization, with a specific focus on enhancing titer and quality

R&D Batch Fermentation

  • 1- 10 L , 5mg-2g yield
  • Quick turnaround times

GMP Batch Fermentation

  • 6 L, 1-5g yield
  • 30L, 5-25g yield

GMP Manufacturing Platform

  • Robust, scalable platform with high reproducibility
  • Automated unit operations
  • Single-use or product dedicated equipment
  • Multi-product facility with high throughput capability for multiple plasmid manufacturing
  • Our process consistently exceeds industry standards for mRNA, AAV, LVV plasmid specification
Upstream Process

Downstream Process Development and Manufacturing

CoJourney produces plasmids with high yield, high purity, low residual, and low endotoxin without the use of chromatography. However, optional anion exchange (AEX) or hydrophobic interaction chromatography (HIC) is available.

Plasmid Specifications

  • High Yield: 50%-70%
  • % Supercoiled: >95%
  • Low Endotoxin: <10 EU/mg
  • 30L batch yield post-purification: 3-15g

Applications

Plasmids That Power Advanced Therapies

High quality plasmids are critical component of a growing number of advanced therapies. Plasmids can be packaged into viral vectors for cell and gene therapies, serve as templates for mRNA transcription, and are administered directly as vaccines or therapeutics.

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Viral Vector Production for Cell and Gene Therapy
mRNA In Vitro Transcription
Plasmid Vector

Analytical Method Development & QC Testing

Verification Of Plasmid Quality

  • Plasmid Platform Assays are readily available
  • In-process monitoring and sampling performed throughout manufacturing process
  • GMP QC labs perform in-process and batch release testing on-site
  • Stability Testing is performed in-house
  • Reference Standard is generated and qualified during PD or GMP runs.
  • QC Team ensures compliance with evolving regulatory requirements
Analytical Services

Ready to get started?

Let’s discuss ways to optimize production of your viral vector.

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Junhui Li

Junhui Li has over 20 years of experience in biologic drug development, manufacturing, and commercialization, with expertise in plasmid, lentiviral, and CAR-T cell manufacturing and quality control, mammalian cell platform development, and process development and GMP production of viral vectors. He previously worked for the international companies ICT and RAAS, where he led research and development, product manufacturing, and supported several IND submissions. Junhui holds both a Ph.D. in Biochemical Engineering and an MBA.

Guang Gao

Guang Gao is an FDA Master Reviewer with over 20 years of FDA regulatory review experience. Guang has reviewed over 800 applications (BLA, PMA, IND, IDE and 510(k)) and has directly contributed to the establishment of rules and regulations for the production of safe and effective medical devices.

Jerry Liu

Jerry Liu is an experienced business development and marketing leader, having served in various roles at Danaher BTG, Cytiva, GE Lifescience, PerkinElmer, and ThermoFisher. With a Doctorate in Business Administration, an EMBA, post-graduate diploma in Finance, and an M.Sc. in Bio-Chemistry, Jerry has a thorough understanding of the CGT market, and the requirements for development and production of high quality CGT products.

Lijun Wang

Lijun Wang has over 20 years of industry experience, with 16 years concentrated in gene therapy process development and GMP manufacturing. She led process development and GMP manufacturing for over 25 batches while employed at Brammer Bio. She also led downstream process development at Applied Genetic Technologies Corporation. Lijun has designed 6 facilities for the manufacture of high quality gene therapy products.